Nested Association Mapping (NAM) Recombinant Inbred Lines
The collection of 5,000 RILs in 25 populations is collectively referred to as the "maize Nested Association Mapping (NAM) population" and was developed by the Molecular and Functional Diversity of the Maize Genome project. NAM is a new approach to the mapping of genes underlying complex traits, in which the statistical power of QTL mapping is combined with the high (potentially gene-level) chromosomal resolution of association mapping. The RILs are "nested" in the sense that they all share a common parent, B73, but each population has a different alternate parent. For further information, please see: 'Maize Nested Association Mapping (NAM) population' at http://www.panzea.org/lit/germplasm.html. Genotypes for the NAM RILs using SNP markers and their genetic map can be found at: http://www.panzea.org/lit/data_sets.html.
Requesting Seeds
Because of the huge number of these lines and the very limited supply of seeds per RIL we're receiving, we will have a very strict policy as to how they are distributed. Essentially, in the public sector, only one request (10 kernels per RIL) will be filled per institute; in the private sector, only one set will be sent per company (world-wide). All subsequent requests, from a company or institute that already was provided with these RILs, will be forwarded to the individual to whom that set was sent.
The scope of this resource makes cooperation essential. Our hope is that this policy will help give access to this important resource to the widest possible number of maize researchers who would like to use it.
Additionally, prior to sending the seeds, we will ask you for an account number for a courier (FedEx would be easiest for us) to cover the shipping costs and ensure that the shipment is tracked while in transit. The 5,000 RILs will be shipped in 3 boxes (18 in x 18 in x 16 in; 46 cm x 46 cm x 41 cm) with a total shipment weight of about 50 lbs (23 Kg). Please let us know which service you would prefer (e.g., overnight, two-day, ground).
Because we did not grow these and the fields where they were grown were not inspected, for requests from outside of the USA, we will need documentation from your government stating that you can receive these without a phytosanitary certificate.
As of now, we are accepting requests for the entire set of 5,000 NAM RILs (please note that we presently have 4,821 of the RILs, the remaining 179 will be sent to those who request sets now, when we receive the seeds [in about one year]). We may accept requests for subsets or individual RILs in future years.
To request the set of 5,000 NAM RILs, please use our Request Form (link on left bar) and input "NAM5000" in the 'Stock ID' field.Public Grow-outs
For those who do not have the resources to grow and maintain these 5,000 stocks, there will be grow-outs of the NAM RILs that will be open for public screening. These will be held as follows:
2008 - Ithaca, NY
2009 - Columbia, MO
2010 - Raleigh, NC
For further information about these grow-outs, please check for updates at http://www.panzea.org/info/NAM_public_growouts.html. Or, contact Jeff Glaubitz.
Inbred parents of NAM RILs
The inbred parents of the NAM RILs are available from NCRPIS. The parents are:
B97 (PI 564682)
CML52 (PI 595561)
CML69 (Ames 28184)
CML103 (Ames 27081)
CML228 (Ames 27088)
CML247 (PI 595541)
CML277 (PI 595550)
CML322 (Ames 27096)
CML333 (Ames 27101)
HP301 (PI 587131)
Il14H (Ames 27118)
Ki3 (Ames 27123)
Ki11 (Ames 27124)
Ky21 (Ames 27130)
M37W (Ames 27133)
M162W (Ames 27134)
Mo18W (PI 550441)
Ms71 (PI 587137)
NC350 (Ames 27171)
NC358 (Ames 27175)
Oh7B (Ames 19323)
Oh43 (Ames 19288)
P39 (Goodman-Buckler accession; Ames 28186)
Tx303 (Ames 19327)
Tzi8 (PI 506246)