Nested Association Mapping (NAM) Recombinant Inbred Lines
The collection of 5,000 RILs in 25 populations is collectively referred to as the "maize Nested Association Mapping (NAM) population" and was developed by the Molecular and Functional Diversity of the Maize Genome project. NAM is a new approach to the mapping of genes underlying complex traits, in which the statistical power of QTL mapping is combined with the high (potentially gene-level) chromosomal resolution of association mapping. The RILs are "nested" in the sense that they all share a common parent, B73, but each population has a different alternate parent. For further information, please see: 'Maize Nested Association Mapping (NAM) population' at http://www.panzea.org/#!phenotypes/c1m50. Genotypes for the NAM RILs using SNP markers and their genetic map can be found at: http://www.panzea.org/#!genotypes/cctl.
Requesting Seeds
Because of the huge number of these lines and the very limited supply of seeds per RIL we're receiving, we will have a very strict policy as to how they are distributed. Essentially, in the public sector, only one request (10 kernels per RIL) will be filled per institute (e.g., site that will grow RILs); in the private sector, only one set will be sent per company (world-wide). All subsequent requests, from a company or institute that already was provided with these RILs, will be forwarded to the individual to whom that set was sent.
Please only request the number of RILs that you are confident you can grow AND propagate during the next growing season. If you cannot propagate all 5,000 RILs in the next growing season, only request the number of sub-populations (approximately 200 RILs per sub-population; see below), that you reasonably expect you can grow and propagate. You can request additional sub-populations to grow in the future.
If you only want to screen the NAM RILs and do not have the ability (or desire) to propagate them, please collaborate with a colleague who will be growing these RILs.
The scope of this resource makes cooperation essential. Our hope is that this policy will help give access to this important resource to the widest possible number of maize researchers who would like to use it.
Additionally, prior to sending the seeds, we will ask you for an account number for a courier (UPS or FedEx would be easiest for us) to cover the shipping costs and ensure that the shipment is tracked while in transit. If you request the entire set of 5,000 RILs, they will be shipped in 3 boxes (18 in x 18 in x 16 in; 46 cm x 46 cm x 41 cm) with a total shipment weight of about 50 lbs (23 Kg). Please let us know which service you would prefer (e.g., overnight, two-day, ground).
Also, to help cover our costs, we will need you to order seed packets and labels for us. The seed packets are small 'coin-type' envelopes (2.5" x 4.25") with a hole through the top of the envelope (including the flap), so that packets can be strung together. A possible source is Midco Global Inc. (Item# 74446). The labels we need are 1" x 2.625". A possible source for these labels is Avery item# 5960.
The seeds will be sent only after we receive the seed packets, labels and UPS or FedEx account number.
Regarding international requests: Because we did not grow these and the fields where they were grown were not inspected, for requests from outside of the USA, we will need documentation from your government stating that you can receive these without a phytosanitary certificate.
To request the set of 5,000 NAM RILs, please use our Request Form (link on left bar) and input "NAM5000" in the 'Stock ID' field.
Alternatively, you can use our shopping cart feature and follow the instructions by clicking: Order Entire set of NAM RILs.
Please make your request two months in advance, especially if you want the entire set.
Please Note: As of now, we presently have 4,835 of the RILs, the remaining 165 will be sent to those who request sets now, when we receive the seeds (in about one year).
Also note: We don't have the inbred parents of these RILs. These parent inbred lines can be requested from the North Central Regional Plant Introduction Station (see below).
Sub-Populations
For those that can grow and propagate some, but not all, of the NAM RILs, you can request sub-pubulations (~200 RILs each) as follows:
Inbred parents of NAM RILs
The inbred parents of the NAM RILs are available from NCRPIS. The parents are:
B97 (PI 564682)
CML52 (PI 595561)
CML69 (PI 690318)
CML103 (PI 690319)
CML228 (PI 690320)
CML247 (PI 595541)
CML277 (PI 595550)
CML322 (PI 690321)
CML333 (PI 690322)
HP301 (PI 587131)
Il14H (PI 690323)
Ki3 (PI 690324)
Ki11 (PI 690325)
Ky21 (PI 690326)
M37W (PI 690327)
M162W (PI 690328)
Mo18W (PI 550441)
Ms71 (PI 587137)
NC350 (PI 690329)
NC358 (PI 690330)
Oh7B (PI 690331)
Oh43 (PI 690332)
P39 (Goodman-Buckler accession; PI 690333)
Tx303 (PI 690334)
Tzi8 (PI 506246)