USDA/ARS/MWA - Soybean/Maize Germplasm, Pathology & Genetics Research Unit
&University of Illinois at Urbana/Champaign - Department of Crop Sciences
S-123 Turner Hall
1102 South Goodwin Avenue
Urbana, IL 61801-4730
|(217) 333-6631 [phone]
(217) 333-6064 [fax]
5,902 seed samples have been supplied in response to 602 requests for 2016. These include 341 requests received from 28 foreign countries. This is the sixth record-breaking year in a row for requests; and greatly exceeded the previous annual record of 519. Interest in reverse genetics tools, such as the UniformMu sequence indexed stocks, continues to grow. Presently, requests for UniformMu stocks represent more than 62% of our total requests. Other popular stock requests include the NAM RILs and other mapping populations, Ac/Ds sequence indexed stocks, Hi II lines, haploid-inducing lines, male sterile cytoplasms, kernel starch quality traits, plant architecture traits, and Maize Inflorescence Project EMS lines.
Approximately 5.5 acres of nursery were grown this summer at the Crop Sciences Research & Education Center located at the University of Illinois. Warm, dry spring weather allowed us to plant both of our crossing nurseries a timely manner, and excellent weather during the growing season allowed for a normal pollination season; no supplemental irrigation was required. There were sufficient stands for a good increase in amost all instances; however, a late July wind storm removed a few rows in their entirety. Those lines will be regrown in our winter greenhouse or next summer’s nursery. Excessive moisture during the late summer and early fall resulted in higher rates of ear rot in susceptible genotypes, but there were no complete losses for any line. Moderate temperatures and low plant stress resulted in excellent yields from most pollinations.
Special plantings were made of several categories of stocks:
1. Plantings were made of donated stocks from the collections of James Brewbaker (br2‑CM104), Hugo Dooner (Bz1‑W22), Susan Gabay-Laughnan (Oh51a mito‑N), Andrea Gallavotti (rte1‑4), Jerry Kermicle (Ga2‑m:102‑13b), Morgan McCaw (Fast Flowering Mini-Maize Line A y1 and R1‑scm2 conversions), Steve Moose (IBM RI M0068), Ron Okagaki (r1‑r:n19 mdr1), Katharine Petsch (rgd1‑lbl1), and others. We expect to receive additional accessions of stocks from maize geneticists within the upcoming year.
2. We are continuing to characterize enhancers of yellow endosperm color from PI accessions of orange endosperm tropical flints and are continuing collaborations to identify the specific gene products associated with previously uncharacterized (or incompletely characterized) white endosperm/albino seedling loci. We are also characterizing a colored pericarp crown variant from a GEM line that is unlinked to p1. Through tests of allelism, we identified a new allele at the v27 locus. We also confirmed the integrity of our A1 sh2 stock. Additional tests of allelism of stocks from our Phenotype Only collection are in progress.
3. Due to lack of personnel, we continue to provide only bare-bones curation of the A-A translocation stocks and other chromosomal aberration stocks that were previously maintained by Janet Day Jackson. We are continuing to grow up recent outcrosses of translocation stocks in our observation fields to score for male and female semisterility. We also had room to increase inversion stocks that had not been grown since 2002.
4. Stocks produced from the NSF project "Regulation of maize inflorescence architecture" (see: http://www.maizegdb.org/MIP/) were grown again this summer. Approximately 250 families of M2 materials that were produced between 2003 and 2007 were grown to increase seed supplies and recover previously observed mutations; this also included previously phenotyped families that had limited seed supplies. In addition, 2,080 families of 2014 and 2015 EMS seed increase materials were grown for adult plant observation and 426 families were screened in sand benches for seedling traits; the materials observed include mutated A619, B73 and Mo17 inbred lines, Mo17xA632 and B73xMo17 hybrid, and various other inbred lines.
5. Critical plantings of a limited number of stocks were made in our greenhouse facilities.
We currently have 14,033 UniformMu sequence indexed stocks, produced by the "Construction of comprehensive sequence indexed transposon resources for maize" project (see: http://www.maizegdb.org/documentation/uniformmu). We have also recently received 58 stocks from the "Maize CellGenomics" project (http://maize.jcvi.org/cellgenomics/). An additional 3,484 lines of tdsg stocks from the "Ac/Ds reverse genetics resource" project (see: http://acdsinsertions.org) were added to our previous collection.
Our IT Specialist has continued to make updates and improvements to our curation tools, which are used to maintain data for our collection. These tools input our public stock data directly into MaizeGDB and our local database to give maize scientists access to up-to-date information about our collection. The current tools are basically in "maintenance mode" while work continues on a new implementation of the curation tools. The current main focus is on unifying databases for common information shared by our group and MaizeGDB personel, which will cut down tremendously the manual manipulation of data sometimes required to make sure our databases stay in sync, and allow us to write unified tools more easily. Maintenance continues on our web site (http://www.uiuc.edu/ph/www/maize).
Agric Sci Res Tech (Plants)
Information Tech Specialist